Vacuolar Localization of Ethylene-Induced Chitinase in Bean Leaves
Open Access
- 1 February 1984
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 74 (2), 442-444
- https://doi.org/10.1104/pp.74.2.442
Abstract
The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular chitinase activity.Keywords
This publication has 9 references indexed in Scilit:
- Ethylene as an Effector of Wound-Induced Resistance to Cellulase in Oat LeavesPlant Physiology, 1982
- Secretory character of yeast chitinase.Journal of Biological Chemistry, 1982
- Isolation and Characterization of Vacuoles from Melilotus alba MesophyllPlant Physiology, 1981
- Regulation of Proteinase Inhibitor Synthesis in Tomato LeavesPlant Physiology, 1981
- Hydrolytic Enzymes in the Central Vacuole of Plant CellsPlant Physiology, 1979
- Immunological Identification of Proteinase Inhibitors I and II in Isolated Tomato Leaf VacuolesPlant Physiology, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Preparation and Purification of Glucanase and Chitinase from Bean LeavesPlant Physiology, 1971
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949