Abstract
Juvenile chinook salmon (Oncorhynchus tshawytscha) memorize odors characteristic of their natal stream, then use these imprinted olfactory cues to return to that same stream years later as sexually mature adults. In a preliminary effort to identify neuroanatomical changes in the salmon olfactory system that may underlie these behavioral capabilities, the structure of the olfactory bulb (OB) was studied at three developmental periods across the life history: in early juvenile development (0.1-4 months of age), in late juvenile development (11-16 months), and as spawning adults (48 months). Using antibodies that selectively label primary olfactory afferents (anti-keyhole limpet hemocyanin), combined with a thionin counterstain, the telencephalon (TEL), OB, and two of the bulb's laminar components, the olfactory nerve-glomerular layer (ONL-GL) and inner cell layer (ICL), could be easily identified. Laminar organization and relative volume (i.e. percent of OB comprised by ONL-GL or ICL) were then compared across groups, and absolute volumes of the OB, ONL-GL, and ICL were compared to that of the TEL at each stage. Three age-related processes were observed across the life history. First, a rapid increase in organizational and structural maturity of the OB was found to be confined to early juvenile life. Second, an increase in OB, ONL-GL, and ICL volume, relative to TEL volume, was found to occur across the entire life history. Lastly, the composition of the OB itself changed, in that an increase in the relative volume of the ONL-GL, and a decrease in that of the ICL, were observed across the life history. Taken together, these results indicate that the OB of young salmon matures dramatically while the fish is still in the freshwater phase of its life history. Then, during migration to the ocean and growth to the adult stage, the volume of the OB, relative to that of the telencephalon, and the relative volume of the input layer of the bulb, both undergo a marked, continuous increase. These changes in the structure, and presumably the function, of the olfactory bulb of salmon may be important in the behavioral phenomena of olfactory imprinting as juveniles and homing as adults.