Chromosome sorting and DNA sequence localization

Abstract

This article focuses on current techniques and possible future developments in chromosome isolation and sorting, and DNA analysis of sorted chromosomes. The strategy of subchromosomal gene mapping by chromosome sorting is outlined and a list of cell lines containing translocated chromosomes is provided which may be used to map genes to a single chromosome with a standard fluorescence activated cell sorter. The usefulness of this and other gene mapping methods for localizing unique DNA sequences and characterizing recombinant DNA libraries constructed from sorted chromosomal DNA is also discussed.