The synthesis of tritiovaline and its incorporation into rat-visceral proteins

Abstract

The synthesis of ([alpha][beta]-H3)-, ([beta]-H3)- and ([alpha]-H3)-valine is reported. The distribution of tritium between the [alpha] and [beta] positions of ([alpha][beta]-H3) valine in this synthesis was unequal and varied in successive preparations. Oxidation of ([alpha][beta]-H3) valine with a variety of reagents to isobutyraldehyde takes place without loss of isotope, indicating retention of [alpha] -hydrogen during the reaction. ([beta]-H3) valine is incorporated into rat visceral proteins at the same rate as ([alpha]-C14) valine. The [alpha]-tritium label of ([alpha][beta]-H3) valine is only partially removed during the incorporation of ([alpha][beta]-H3) valine into rat visceral proteins.