Silicic Acid Chromatography of Lipids of Whole Human Blood.

Abstract

Normal human blood and the blood from a xanthomatosis patient was extracted at -20[degree]C to yield lipids which were fractionated by silicic acid chromatography. Each yielded at least 20 distinct fractions, representing different classes of compounds, identified by paper chromatography, specific chemical reactions and infra-red spec-trophotometry. Somewhat excessive amounts of cholesterol and cholesterol esters were found in the lipids from the xanthomatosis patient but qualitative difference in the amino acid-containing lipids are not considered significant since the normal variation in these components is not known.