The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt
- 1 September 1976
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 8 (5), 501-506
- https://doi.org/10.1007/bf01003839
Abstract
This article describes the use of a microdensitometer for the measurement of BPST formazan in tissue sections. BPST is a new tetrazolium salt, 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride, which produces a single, well-defined formazan, and is thus easily quantified. The formazan gives an excellent localization, since BPST was originally designed for ultrastructural work. Activities are expressed in absolute units as n moles hydrogen/mm3, and are thus directly comparable with standard biochemical data.This publication has 10 references indexed in Scilit:
- The quantification of formazans in tissue sections by microdensitometry. I. The use of neotetrazolium chlorideJournal of Molecular Histology, 1976
- Tetrazolium Salts and FormazansProgress in Histochemistry and Cytochemistry, 1976
- [Thin layer chromatographic and spectrophotometric testing of tetrazolium salts and their formazans for their use as quantitative redox indicators].1975
- Studies on the reduction of tetrazodium saltsHistochemistry and Cell Biology, 1974
- Studies on the reduction of tetrazolium saltsHistochemistry and Cell Biology, 1973
- Studies on the reduction of tetrazolium saltsHistochemistry and Cell Biology, 1973
- NONOSMIOPHILIC TETRAZOLIUM SALTS THAT YIELD OSMIOPHILIC, LIPOPHOBIC FORMAZANS FOR ULTRASTRUCTURAL LOCALIZATION OF DEHYDROGENASE ACTIVITYJournal of Histochemistry & Cytochemistry, 1972
- The use of a new grade of polyvinyl alcohol for stabilising tissue sections during histochemical incubationsHistochemistry and Cell Biology, 1971
- Aspects of tetrazolium salt reduction relevant to quantitative histochemistryHistochemistry and Cell Biology, 1970