CELLULAR LOCALIZATION OF ANTI-DNP-PLL AND ANTICONVEYOR ALBUMIN ANTIBODIES IN GENETIC NONRESPONDER GUINEA PIGS IMMUNIZED WITH DNP-PLL ALBUMIN COMPLEXES

Abstract

Genetic nonresponder guinea pigs incapable of an immune response to DNP-PLL [2,4-dinitro-phenyl poly-L-lysine] alone were immunized with DNP-PLL completed to ovalbumin or bovine serum albumin. Under these circumstances the animals produce both anti-DNP-PLL antibodies directed against the conveyor albumin. Thus immune response to DNP-PLL complexed to conveyer albumin molecules can serve as a simple model of hapten-carrier relationships. To determine if these 2 types of antibody are synthesized by the same or by different plasma cells, a combination of a double immunofluorescent technique and radioautographic localization of radioactive antigen was used. The anti-DNP-PLL antibodies and the antibodies against the carrier albumin molecule were produced in separate cells. No cell-producing antibodies with both specificities were detected out of 526 cells studied.