Phage type and DNA plasmid profile ofSalmonella typhimuriumisolates in the area of Isernia, Italy

Abstract

SUMMARY: Thirty-eightSalmonella typhimuriumstrains isolated from December 1987 to March 1988 in Isernia, Central Italy, were characterized on the basis of their phage type, resistance to antimicrobials and plasmid profiles. According to their phage types, the isolates could be assigned to one of six groups, the prevalent one being PT 195 which accounted for 73·6% of isolates.On the basis of their plasmid content, the isolates could be assigned to one of ten groups. The prevalent plasmid profile (60·0; 6·0; 4·3; 4·0; 3·2 megadaltons) was found in 60·4% of isolates.All the isolates from a particular food (salsicce), and as most of isolates from humans who had consumed this food belonged to phage type 195 and were of the same plasmid profile.The combined use of phage typing and DNA plasmid analysis proved to be a useful tool in identifying epidemiologically related isolates in this investigation.