Expression in Escherichia coli of catalytically active phenylalanine ammonia‐lyase from parsley
Open Access
- 4 December 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (2), 335-338
- https://doi.org/10.1016/0014-5793(89)81687-4
Abstract
In parsley (Petroselinum crispum), phenylalanine ammonia‐lyase (PAL) is encoded by 4 structurally similar genes. The nucleotide sequence of a near full‐length cDNA and the deduced amino acid sequence of PAL‐4 are presented and compared with the corresponding sequences of PAL‐1, a previously described representative of the gene family. Transformation of Escherichia coli cells with PAL‐1 or PAL‐4 cDNA yielded catalytically active PAL, suggesting that the catalytic center of the enzyme is formed spontaneously rather than by a plant‐specific mechanism.Keywords
This publication has 16 references indexed in Scilit:
- Physiology and Molecular Biology of Phenylpropanoid MetabolismAnnual Review of Plant Physiology and Plant Molecular Biology, 1989
- Phenylalanine ammonia-lyase gene organization and structurePlant Molecular Biology, 1989
- Primary structures and catalytic properties of isoenzymes encoded by the two 4‐coumarate: CoA ligase genes in parsleyEuropean Journal of Biochemistry, 1988
- Several “pathogenesis-related” proteins in potato are 1,3-β-glucanases and chitinasesProceedings of the National Academy of Sciences, 1988
- Vectors for selective expression of cloned DNAs by T7 RNA polymeraseGene, 1987
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology, 1986
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Light‐Induced Enzyme Synthesis in Cell Suspension Cultures of Petroselinum hortenseEuropean Journal of Biochemistry, 1976
- Light-induced changes of enzyme activities in parsley cell suspension culturesArchives of Biochemistry and Biophysics, 1975