Sensitive Amplified Immunoenzymometric Assays (IEMA) for Human Insulin and Intact Proinsulin
- 1 January 1992
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 30 (1), 27-32
- https://doi.org/10.1515/cclm.1992.30.1.27
Abstract
Immunoenzymometric assays (IEMAs) for human insulin and intact proinsulin were developed using the amplification system developed by Johannsson et al. (Clin. Chim. Acta 148 (1985) 119-124) for the detection of the enzyme alkaline phosphatase. The detection limit of the assays was 0.8 pmol/l for proinsulin and 0.8 pmol/l for insulin whereas it was 1.8 pmol/l and 2.3 pmol/l respectively for the homologous immunoradiometric assays (IRMA). These assays are superior to immunoradiometric assays in terms of sensitivity, shelf-life of the labelled antibody and suitability for automation.Keywords
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