CopA: An Escherichia coli Cu(I)-translocating P-type ATPase
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- 18 January 2000
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 97 (2), 652-656
- https://doi.org/10.1073/pnas.97.2.652
Abstract
The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae. Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.Keywords
This publication has 30 references indexed in Scilit:
- Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPaseJournal of Biological Chemistry, 1999
- Molecular Mechanisms of Copper HomeostasisBiochemical and Biophysical Research Communications, 1999
- Functional Analysis of the N-terminal CXXC Metal-binding Motifs in the Human Menkes Copper-transporting P-type ATPase Expressed in Cultured Mammalian CellsPublished by Elsevier ,1999
- Undetectable Intracellular Free Copper: The Requirement of a Copper Chaperone for Superoxide DismutaseScience, 1999
- A Zn(II)-translocating P-type ATPase from Proteus mirabilisBiochemistry and Cell Biology, 1998
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997
- Zinc(II) tolerance in Escherichia coli K‐12: evidence that the zntA gene (o732) encodes a cation transport ATPaseMolecular Microbiology, 1997
- Helicobacter pylorigeneshpcopAandhpcopPconstitute a cop operon involved in copper exportFEMS Microbiology Letters, 1996
- Identification of a protein required for disulfide bond formation in vivoCell, 1991
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970