Interaction of fibrinogen with surfaces of end‐group‐modified polyurethanes: A surface‐specific sum‐frequency‐generation vibrational spectroscopy study
- 2 August 2002
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 62 (2), 254-264
- https://doi.org/10.1002/jbm.10075
Abstract
Fibrinogen adsorption on polyurethanes with different surface-modifying end groups (SMEs) has been studied with sum-frequency-generation vibrational spectroscopy (SFG). The results show very different protein adsorption properties for different SMEs on the same backbone polymer. Fibrinogen binds weakly on the hydrophilic backbone of a poly(dimethyl siloxane) (PDMS)-modified polyurethane surface but leaves the hydrophobic PDMS part untouched. On sulfonate end-group-modified (SO) polyurethane surfaces, fibrinogen adsorbs well. However, on poly(ethylene oxide) (PEO)-modified surfaces, it adsorbs poorly. The protein-resistant character of PEO is probably due to steric repulsion. This work demonstrates the utility of SFG in the study of protein adsorption on polymeric biomaterials at the molecular level and the ability of SMEs to mediate protein adsorption. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 254–264, 2002Keywords
This publication has 37 references indexed in Scilit:
- Conformational aspects of model chromatographic surfaces studied by sum-frequency generationAnalytica Chimica Acta, 1999
- Molecular Characterization of Polymer and Polymer Blend Surfaces. Combined Sum Frequency Generation Surface Vibrational Spectroscopy and Scanning Force Microscopy StudiesAccounts of Chemical Research, 1999
- Surface Studies of Polymer Blends by Sum Frequency Vibrational Spectroscopy, Atomic Force Microscopy, and Contact Angle GoniometryThe Journal of Physical Chemistry B, 1998
- Phosphatidylcholine Monolayer Structure at a Liquid−Liquid InterfaceJournal of the American Chemical Society, 1998
- Investigations of the Structure and Hydrogen Bonding of Water Molecules at Liquid Surfaces by Vibrational Sum Frequency SpectroscopyThe Journal of Physical Chemistry B, 1998
- Behavior of proteins at interfacesCurrent Opinion in Colloid & Interface Science, 1996
- Investigation of Surfactant Conformation and Order at the Liquid−Liquid Interface by Total Internal Reflection Sum-Frequency Vibrational SpectroscopyThe Journal of Physical Chemistry, 1996
- Effect of sulfonation of segmented polyurethanes on the transient adsorption of fibrinogen from plasma: Possible correlation with anticoagulant behaviorJournal of Biomedical Materials Research, 1992
- Infrared spectroscopic studies of time-dependent changes in fibrinogen adsorbed to polyurethanesLangmuir, 1991
- Dimeric half-molecules of human fibrinogen are joined through disulfide bonds in an antiparallel orientationBiochemistry, 1983