Virus-induced amyloidosis in scrapie involves a change in covalent linkages in the preamyloid
- 1 March 1991
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 118 (1-2), 127-131
- https://doi.org/10.1007/bf01311308
Abstract
Preparations of the preamyloid and the amyloid protein from normal and scrapie hamster brains show different solubilization behaviours towards Triton X-114 extraction. The normal isoform is completely extractable from microsomal membranes by the detergent, whereas the pathological one is not. Both forms can be isolated using preparative SDS electrophoresis as the final step in order to remove all non-covalently associated materials. After removal of the SDS these purified proteins retain their solubility differences against Triton X-114. This demonstrates that at least one distinct modification of the preamyloid protein — which has to be covalent in nature — must have occurred to account for the strong aggregation tendency of the pathological isoform.Keywords
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