FOWL ANTIBODY .X. PURIFICATION AND PROPERTIES OF AN ANTIBODY TO 2,4-DINTIROPHENYL GROUP
- 1 January 1968
- journal article
- research article
- Vol. 14 (1), 61-+
Abstract
A preparation of fowl antibody to the 2,4-dinitro-phenyl group (DNP), made from antisera to DNP-bovine gamma-globulin by dissociation of a specific precipitate, was more than 90% precipitable by DNP-ovalbumin. This antibody with an approximate molecular weight of 165,000 and the immunoelectrophoretic properties of gammaG-globulin, precipitated with antigen only in 8% NaCl. It fixed fowl complement but did not agglutinate tanned fowl red cells coated with DNP-protein. It had no skin sensitizing activity. Its amino-acid composition was significantly different from that of mammalian antibodies. Some of the properties of another DNP-specific antibody that appeared later in immunzation are also described.This publication has 15 references indexed in Scilit:
- FOWL ANTIBODY .9. DIFFERENT RESPONSES TO HAPTEN AND CARRIER MOIETIES OF 2,4-DINITROPHENYL-BOVINE GAMMA-GLOBULIN1968
- Identification Of Two Fowl β Macroglobulins with Precipitating Antibody ActivityNature, 1966
- ImmunoglobulinsAnnual Review of Biochemistry, 1966
- Location of Amino Acid Differences in the Subunits of Three Rabbit Antibodies*Biochemistry, 1966
- Aggregation of chicken γG immunoglobulin in 1.5 M sodium chloride solutionBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- CHARACTERISTICS OF 19S AND 7S RESPONSE TO BACTERIOPHAGE OX174 IN FOWL1966
- Some physical-chemical properties of chicken γ-globulins and their pepsin and papain digestion productsImmunochemistry, 1966
- PROPERTIES OF PAPAIN-DIGESTED CHICKEN 7 S GAMMA-GLOBULIN1965
- Molecular weight of Rabbit Gamma-GlobulinNature, 1962
- FOWL ANTIBODY .4. ESTIMATION OF HAEMOLYTIC FOWL COMPLEMENT1962