Met-enkephalin: Rapid separation from brain extracts using high-pressure liquid chromatography, and quantitation by binding assay
- 1 June 1978
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 3 (3), 367-372
- https://doi.org/10.1007/bf00965581
Abstract
Met-enkephalin can be rapidly separated with high recovery from leu-enkephalin, β-endorphin, and enkephalin metabolites by high-pressure liquid chromatography on a Dupont SCX column. The technique permits separation of endogenous enkephalins from brain extracts for measurement by opiate-receptor binding assay, immunoassay, or for study of the incorporation of radiolabeled amino acids into enkephalin. Using this technique, met-enkephalin was separated from striatal extracts of rats killed by focused microwave irradiation and quantitated by the opiate-receptor binding assay. The concentration of met-enkephalin in the column eluate was 1.3 μg/g tissue. This value is comparable to that obtained by radioimmunoassay without purification of the extracts.Keywords
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