Rat Brain Synaptosomes Prepared by Phase Partition
- 1 December 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 55 (6), 1841-1849
- https://doi.org/10.1111/j.1471-4159.1990.tb05766.x
Abstract
Synaptosomes from rat forebrain can easily be isolated by combining centrifugation with partition in an aqueous two-phase system composed of dextran T500 and polyethylene glycol 4000 in which synaptosomes have an extreme affinity for the upper phase. The fraction thus obtained has been characterized by electron microscopy and biochemical markers for synaptosomes and some other cell components. The contamination by microsomes, free mitochondria, and myelin was 4.4, 3.2, and 0.1%, respectively. The morphometric analysis of the electron micrographs shows that > 60% of the structures are synaptosomes. This preparation of the isolation procedure is remarkably short (< 1 h), formance as assayed by their respiratory activities and ATP level in the absence and presence of depolarizing agents. Synaptosomes prepared by phase partition release the neurotransmitter glutamate in a Ca2+-dependent manner. The duration of the isolation procedure is remarkably short (< 1 h), no ultracentrifuge is required, and the method can be applied for small- or large-scale preparations.Keywords
This publication has 25 references indexed in Scilit:
- Calcium‐Dependent and‐Independent Release of Glutamate from Synaptosomes Monitored by Continuous FluorometryJournal of Neurochemistry, 1987
- Synaptosomal bioenergeticsEuropean Journal of Biochemistry, 1986
- Influence of the conformational state on the isoelectric points of rat brain synaptosomes, mitochondria and mitoplastsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1984
- Rapid Preparation of Synaptosomes from Mammalian Brain Using Nontoxic Isoosmotic Gradient Material (Percoll)Journal of Neurochemistry, 1984
- Highly purified mitochondria from rat brain prepared by phase partitionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- ISOLATION AND PARTIAL CHARACTERIZATION OF RAT BRAIN SYNAPTIC PLASMA MEMBRANES1Journal of Neurochemistry, 1974
- Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterizationBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEXJournal of Neurochemistry, 1969
- A simple method for isolation of nerve-ending particles from rat brainBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- ON THE ISOLATION OF NERVE ENDINGS AND SYNAPTIC VESICLESThe Journal of cell biology, 1961