Cultural method for large-scale screening for Chlamydia trachomatis genital infection.
Open Access
- 1 July 1981
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 34 (7), 712-718
- https://doi.org/10.1136/jcp.34.7.712
Abstract
Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be examined concurrently. It is sufficiently sensitive to form the basis of a chlamydial culture service for patients attending Sexually Transmitted Diseases (STD) Clinics.This publication has 12 references indexed in Scilit:
- Value and feasibility of screening women attending STD clinics for cervical chlamydial infections.Sexually Transmitted Infections, 1980
- The rôle of Chlamydia trachomatis in genital-tract and associated diseases.Journal of Clinical Pathology, 1980
- Techniques for Culturing and Determining Antimicrobial Susceptibility of Chlamydia TrachomatisArchives of Andrology, 1980
- Prospective Study of Maternal and Infantile Infection with Chlamydia trachomatisPediatrics, 1979
- The need for a chlamydial culture service.Sexually Transmitted Infections, 1979
- Fifteen-Month Follow-up Study of Women Infected withChlamydia trachomatisNew England Journal of Medicine, 1979
- Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents)Epidemiology and Infection, 1976
- Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract.Journal of Clinical Pathology, 1975
- A comparison of staining techniques for demonstrating group A chlamydia in tissue culture.1975
- The isolation of Chlamydia subgroup A (Chlamydia trachomatis) in irradiated McCoy cells.1974