Cyclic Nucleotides and Growth Control in Cultured Mouse Cells: Correlation of Changes in Intracellular 3′:5′cGMP Concentration with a Specific Phase of the Cell Cycle
Open Access
- 1 December 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (12), 4920-4924
- https://doi.org/10.1073/pnas.71.12.4920
Abstract
The commencement of cell growth following serum addition to quiescent cultures of mouse fibroblasts is preceded by transient changes in intracellular concentrations of cAMP and cGMP. By artificial depletion of the culture medium for different nutrients, cell growth can be reversibly arrested in various phases of the cell cycle. Here it is shown that the major cGMP increases are only observed when cultures which are arrested in the G0 phase are stimulated to grow or when synchronized growing cells pass through the G1 phase. In addition to its concomitant decrease, cAMP exhibits rhythmic changes during the cell cycle. This suggests that the increase in cGMP could act as a specific signal for movement of cells out of the G0 or G1 phase of the cell cycle by activating the pleiotypic and mitogenic program of the cell.Keywords
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