A Direct Plating Method for Monitoring the Contamination ofListeria monocytogenesin silage
- 12 January 1992
- journal article
- research article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 39 (1-10), 513-518
- https://doi.org/10.1111/j.1439-0450.1992.tb01200.x
Abstract
Twenty‐two silage samples were analyzed for the presence of L. monocytogenes using five Listeria selective plating media, with and without previous selective enrichment step. L. monocytogenes was recovered from 3 samples by both procedures, but direct plating allowed the quantification of Listeria population. Two of these positive samples were implicated in outbreaks of listeriosis in sheep; the L. monocytogenes population in these samples was about 106cells/g. The L. monocytogenes population in the other positive sample was 103cells/g. Direct isolation of L. monocytogenes was only possible from LPM, PALCAM and LSAMm media. MOX and LSM media were not selective enough to allow direct Listeria isolation. In our hands, LSAMm was the most suitable plating medium for the direct isolation and specific quantification of L. monocytogenes from silage employing a red blood cells overlay technique.Keywords
This publication has 18 references indexed in Scilit:
- Overlay technique for direct detection and identification of haemolytic Listeria on selective plating mediumZeitschrift für Lebensmittel-Untersuchung und Forschung, 1990
- A technique for the direct identification of haemolytic-pathogenic listeria on selective plating mediaLetters in Applied Microbiology, 1989
- Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp.International Journal of Food Microbiology, 1989
- A selective differential medium for the isolation of Listeria monocytogenesLetters in Applied Microbiology, 1989
- Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal originInternational Journal of Food Microbiology, 1988
- Listeria monocytogenes in Raw Milk: Detection, Incidence, and PathogenicityJournal of Food Protection, 1987
- Rapid quantitative assessment of the distribution of Listeria in silage implicated in a suspected outbreak of listeriosis in calvesPublished by Wiley ,1986
- Ovine listerial encephalitis: analysis, hypothesis and synthesisPublished by Wiley ,1985
- Septicaemia, encephalitis and abortions in a housed flock of sheep caused by Listeria monocytogenes type 1/2Published by Wiley ,1985
- Isolation of Listeria monocytogenes from Oat SilageScience, 1960