Mouse Monoclonal Anti-HBs and Its Use in the Screening of Donated Blood by Elisa
- 1 March 1984
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 46 (3), 165-174
- https://doi.org/10.1111/j.1423-0410.1984.tb00070.x
Abstract
A method is described for the production of mouse monoclonal antibodies to HBsAg [hepatitis B surface antigen]. Four clones produced antibodies directed at the a and 1 at the d determinant of HBsAg. These antibodies were conjugated to horseradish peroxidase and used in ELISA [enzyne-linked immunosorbent assay] for the detection of HBsAg in donated blood. Antibody C10 produced conjugate which rendered the ELISA as sensitive as a commercially obtainable immunoradiometric assay with which it was compared. Conditions for this assay were optimized and it may be used as a rapid (1 h) test for detecting HBsAg-positive blood. It is practicable to read this ELISA by eye instead of photometrically and it can be thus used under field conditions or in emergencies.This publication has 18 references indexed in Scilit:
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