Abstract
Using 13C NMR spectroscopy the major sterols of the unicellular chlorophyte D. tertiolecta were ergosterol and a closely related C29 trienol. Apparently the absence of a cell wall in D. tertiolecta does not significantly affect the type of sterol produced since other unicellular chlorophytes which possess a cell wall produce similar sterols containing an unsaturated side chain and a 24[R]-alkyl substituent.