Abstract
A technique was developed for germinating teliospores of the bean rust fungus, (U. phaseoli var. typica), which allowed all 5 spore stages to develop on bean [Phaseolus vulgaris] plants. Teliospores germinated on water-agar plates, beginning about 3-4 wk after a brief washing. Pycnia were obtained by inverting those plates over young bean plants in a greenhouse. Pycniospores were transferred between pycnia with sterile toothpicks. Aeciospores were collected, and host leaves were inoculated and incubated using the same methods as were used for urediospores. The technique has worked with many collections of the bean rust fungus that produced telia readily.
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