Localization of PURA, the gene encoding the sequence-specific single-stranded-DNA-binding protein Purα, to chromosome band 5q31

Abstract

Purα (PurA) is a sequence-specific single-stranded-DNA-binding protein implicated in control of both DNA replication and transcription. We have localized the Purα gene (PURA) to human chromosome band 5q31 by fluorescence in situ hybridization with a 16-kb genomic probe together with hybridization of a cDNA probe to blots of DNA from human-hamster cell lines containing individual human chromosomes. Sequences with homology to the PURA locus are also present at 6ql4. The 5q31 locus is frequently deleted in myelogenous leukemias and other cancers.