Peptidoglycan biosynthesis by preparations from Bacillus licheniformis: cross-linking of newly synthesized chains to preformed cell wall
- 1 June 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 139 (3), 781-784
- https://doi.org/10.1042/bj1390781
Abstract
A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by 14C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.Keywords
This publication has 9 references indexed in Scilit:
- The direction of glycan synthesis in a bacterial peptidoglycanBiochemical Journal, 1973
- Role of the Penicillin-Sensitive Transpeptidation Reaction in Attachment of Newly Synthesized Peptidoglycan to Cell Walls of Micrococcus luteusProceedings of the National Academy of Sciences, 1972
- Biosynthesis of peptidoglycan by a cell wall preparation of Staphylococcus aureus and its inhibition by penicillinBiochemical and Biophysical Research Communications, 1972
- Autolytic Enzymes in Growth of BacteriaNature, 1971
- Specificity of combination between mucopeptide precursors and vancomycin or ristocetinBiochemical Journal, 1969
- BIOSYNTHESIS OF PEPTIDOGLYCAN OF BACTERIAL CELL WALLS .11. FORMATION OF ISOGLUTAMINE AMIDE GROUP IN CELL WALLS OF STAPHYLOCOCCUS AUREUS1968
- The cell wall of Bacillus licheniformis N.C.T.C. 6346. Isolation of low-molecular-weight fragments from the soluble mucopeptideBiochemical Journal, 1968
- The purification and properties of two staphylolytic enzymes from Streptomyces griseusBiochemical Journal, 1967
- Electrophoretic Mobilities of Peptides on Paper and their Use in the Determination of Amide GroupsNature, 1966