Requirement of the human chromosome 11 long arm for replication of herpes simplex virus type 1 in nonpermissive Chinese hamster x human diploid fibroblast hybrids
- 1 March 1982
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 7 (2), 171-191
- https://doi.org/10.1007/bf01567656
Abstract
Somatic cell hybrids between Chinese hamster (CH) lung cells (V79/380-6), nonpermissive for productive infection by herpes simplex type 1 (HSV-1), and permissive human diploid cells support productive HSV-1 infection as long as they retain human chromosome 11. Human chromosome 3 has been reported to complement nonpermissivity in (CH) Don cells (1). Intraspecies hybrids between Don/a3 and V79/380-6 cells, however, did not support HSV-1 replication, indicating lack of complementation. The block in both nonpermissive CH cell lines was determined to involve a step beyond replication of the parental viral DNA. In cell hybrids between nonpermissive Don/a23 cells and human fibroblasts containing a t(11;15) (p11;p12) translocation, HSV-1 production was dependent solely on the presence of either human chromosome 11 or the der(11) (p11→qter) translocation product containing the long arm of chromosome 11. Chromosome 3 was excluded by a discordancy rate of 59%. We conclude that the long arm of human chromosome 11 carries one or more genes coding for host functions necessary for the production of progeny HSV-1 DNA.This publication has 30 references indexed in Scilit:
- The β-globin gene is on the short arm of human chromosome 11Nature, 1980
- IntroductionCytogenetic and Genome Research, 1979
- Herpes Simplex Virus Nucleic Acid Synthesis Following Infection of Non-permissive XC CellsJournal of General Virology, 1978
- A gene (Bevi) on human chromosome 6 is an integration site for baboon type C DNA provirus in human cellsCell, 1978
- Herpes Simplex Virus Infection of in vitro Cultured Neuronal Cells (Mouse Neuroblastoma C 1300 Cells)Journal of General Virology, 1978
- Cell-free synthesis of herpes simplex virus DNA: conditions for optimal synthesisBiochemistry, 1977
- A new genetic locus, bevi, on human chromosome 6 which controls the replication of baboon type C virus in human cellsCell, 1977
- Assignments of the human genes for lactate dehydrogenase-A and thymidine kinase to specific chromosomal regionsCytogenetic and Genome Research, 1975
- Regional localization of the human genes for malate dehydrogenase-1 and isocitrate dehydrogenase-1 on chromosome 2 by interspecific hybridization using human cells with the balanced reciprocal translocation t(1;2)(q32;q13)Cytogenetic and Genome Research, 1975
- Human chromosome 19 carries a poliovirus receptor geneCell, 1974