Induction of Protein Kinase C Translocation and Cell Differentiation in ML-1 Human Myeloblastic Leukemic Cells by Tumor Necrosis Factor-α, Transforming Growth Factor-β, or Tetradecanoylphorbol Acetate

Abstract

Tumor necrosis factor-α and transforming growth factor-β, like 12-O-tetradecanoylphorbol 13-acetate, induce differentiation of ML-1 human myeloblastic leukemia cells along the monocyte path. As measured at 5 min following exposure of the cells to either of these agents, extensive translocation of protein kinase C from the cytosolic to the membrane fraction occurred. A correlation was observed to exist between protein kinase C translocation, cell differentiation, and cessation of cell growth induced by transforming growth factor-β and tumor necrosis factor-α.