Preservation of Cell Cultures at 4 C

Abstract

A method is described whereby stable strains of cultured cells may be preserved at 4[degree] C for periods in excess of 6 weeks. If sufficient time is allowed for recovery of cultures the process may be repeated many times. Essentials of the method are that the cells be in good condition when refrigerated and that no replacement in the nutrient fluid be made during the storage period and for several days after the cells are returned to 37[degree] C.