Antiglycolipid Antibodies in Normal and Pathologic Human Sera and Synovial Fluids

Abstract

Antiglycolipid antibodies were measured in normal and pathologic sera and synovial fluids by means of a modified microplate method of complement‐mediated immune lysis of fluorescent dye‐trapped liposomes. All sera of normal subjects had antibodies against globopentaosylceramide (IV³ GalNAcGbOse₄Cer), ganglioside G_MI, gangliotriaosylceramide, gangliotetraosylceramide, and galactosylneolactotetraosylceramide antigens. Most sera of normal subjects had antibodies against lactotriaosylceramide, N‐glycolylneuraminosyl‐neolactotetraosylceramide (NeuGcnLcOse₄Cer), G_M3 ganglioside with N‐glycolylneuraminic acid (NeuGcG_M3) and G_DIa antigens. Differences of titers against IV³GalNAcGbOse₄Cer, neolactotetraosylceramide, NeuGcG_M3 and NeuGcnLcOse₄Cer antigens were observed between sera of normal subjects and pathologic sera from cases of leukemias, lymphomas, several autoimmune diseases and liver diseases.