Entrapment of Enzymes and Microorganisms in Synthetic Cross-linked Polymers and their Application in Column Techniques.

Abstract

A simple technique for the entrapment of enzymes and microorganisms in synthetic cross-linked polymers is described. The obtained gels containing enzyme or microorganism were granulated and used in continuous column processes. The activity of gel granules containing orsellinic acid decarboxylase was measured using Warburg technique and found to be as high as 30% in relation to free enzyme. Leakage of enzyme from the gel matrix was negligble over a period of 14 days.