Intracellular chloride activities and active chloride absorption in the intestinal epithelium of the winter flounder

Abstract

Intracellular chloride activities, (Cl) _c , and the electrical potential difference across the mucosal membrane, ψ_mc, were determined in the isolated small intestine of the winter flounder, using Cl-selective and conventional (KCl-filled) microelectrodes. In the presence of a Na-containing buffer ψ_mc averages −69mV and (Cl) _c averages 24mM, a value that is 3.4 times that predicted for an equilibrium distribution across the mucosal membrane. On the other hand, when the tissue is then perfused with Na-free buffer, (Cl) _c slowly falls to a value that does not differ significantly from that predicted for an equilibrium distribution, and ψ_mc depolarizes significantly. Finally, when the tissue is again bathed in the Na-containing buffer, (Cl) _c rapidly returns to a value well above equilibrium. These results, together with those of Frizzellet al. (J. Membrane Biol. 46:27, 1979), provide direct evidence that: (1) Cl is accumulated against its electrochemical potential difference (32mV) by this tissue, and (2) this accumulation is coupled to and energized by the entry of Na down its steep electrochemical potential difference.