Characteristics of an Acid Protease from Maize Endosperm
- 1 March 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 53 (3), 449-452
- https://doi.org/10.1104/pp.53.3.449
Abstract
An assay has been developed to measure protease activity in endosperm extracts of maize seeds. With hemoglobin as substrate, the enzyme(s) has a pH optimum of 3.8 and a temperature optimum of 46 C. It also degrades gliadin, edestin, bovine serum albumin, and partially hydrolyzed zein and glutelin under standard assay conditions. The enzyme(s) has endopeptidase activity with all substrates tested. When undenatured zein and glutelin are suspended in an agar gel, both are efficiently degraded. Using this assay, the protease activity increases from day 3 to day 8 after inhibition and then declines.Keywords
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