Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation
- 1 March 1980
- journal article
- research article
- Published by Springer Nature in Journal of Neural Transmission
- Vol. 47 (1), 53-60
- https://doi.org/10.1007/bf01256639
Abstract
The effect of l-noradrenaline and vanadate on the activity of Na+K+-activated ATPase was studied on synaptosomal brain cortex preparation. Using neutron activation analysis it was found that the rat cerebral cortex synaptosomal preparation contains 0.16μM. vanadium. The concentration of vanadium needed to reduce enzyme activity by 50% proved to be 2×10−6 M. Evidence has been provided that the increase by noradrenaline of enzyme activity in synaptosomal preparation depends on the presence of an inhibitory contaminant in commercial ATP preparations. In homogenate, however, noradrenaline was able to enhance enzyme activity even when vanadium-free ATP was used. This fact indicates that noradrenaline removes the inhibitory effect of cytoplasmic factor thereby stimulating enzyme activity.Keywords
This publication has 23 references indexed in Scilit:
- Vanadate selectively inhibits the K0+-activated Na+ efflux in squid axonsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Effects of α- and β-adrenergic blocking agents on the biogenic amine stimulated (Na+K+) ATPase of rat cerebral cortical synaptosomal membraneGeneral Pharmacology: The Vascular System, 1978
- Vanadate inhibits the red cell (Na+, K+) ATPase from the cytoplasmic sideNature, 1978
- Commercial ATP containing traces of vanadate alters the response of (Na+ + K+)ATPase to external potassiumNature, 1978
- Stimulation of cerebral cortical synaptosomal Na-K-ATPase by biogenic aminesCanadian Journal of Physiology and Pharmacology, 1977
- THE EFFECTS OF OUABAIN AND THE ACTIVATION OF NEURAL MEMBRANE ATPase BY BIOGENIC AMINESJournal of Neurochemistry, 1976
- An improved method for the preparation of synaptosomal fractions in high purityBrain Research, 1975
- Nerve terminal ATPase as possible trigger for neurotransmitter releaseNature, 1975
- Ascorbic acid-like effect of the soluble fraction of rat brain on adenosine triphosphatases and its relation to catecholamines and chelating agentsBiochemical Pharmacology, 1974
- Effects of tetrabenazine and of chlorpromazine on submitochondrial adenosine triphosphatases of rat brain in the presence of the soluble fractionBiochemical Pharmacology, 1973