Glutamate Stimulation of [3H]Dopamine Release from Dissociated Cell Cultures of Rat Ventral Mesencephalon
- 1 April 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 52 (4), 1300-1310
- https://doi.org/10.1111/j.1471-4159.1989.tb01879.x
Abstract
In dissociated cell cultures of fetal rat ventral mesencephalon preloaded with [3H]dopamine, glutamate (10-5-10-3M) stimulated the release of [3H]dopamine. Glutamate stimulation of [3H]dopamine release was Ca2+ dependent and was blocked by the glutamate antagonist, cis-2,3-piperidine dicarboxylic acid. Glutamate stimulation of [3H]dopamine release was not due to glutamate neurotoxicity because (1) glutamate did not cause release of a cytosolic marker, lactate dehydrogenase, and (2) preincubation of cultures with glutamate did not impair subsequent ability of the cells to take up or release [3H]dopamine. Thus, these dissociated cell cultures appear to provide a good model system to characterize glutamate stimulation of dopamine release. Release of [3H]dopamine from these cultures was stimulated by verat-ridine, an activator of voltage-sensitive Na+ channels, and this stimulation was blocked by tetrodotoxin. However, glutamate stimulated [3H]dopamine release was not blocked by tetrodotoxin or Zn2+. Substitution of NaCl in the extracellular medium by sucrose, LiCl, or Na2SO4 had no effect on glutamate stimulation of [3H]dopamine release; however, release was inhibited when NaCl was replaced by choline chloride or N-methyl-d-glucamine HCl. Glutamate-stimulated [3H]-dopamine release was well maintained (60-82% of control) in the presence of Co2+, which blocks Ca2+ action potentials, and was unaffected by the local anesthetic, lidocaine. These results are discussed in terms of the receptor and ionic mechanisms involved in the stimulation of dopamine release by excitatory amino acids.Keywords
This publication has 57 references indexed in Scilit:
- In Vivo Release of Endogenous Amino Acids from the Rat Striatum: Further Evidence for a Role of Glutamate and Aspartate in Corticostriatal NeurotransmissionJournal of Neurochemistry, 1986
- Glutamate neurotoxicity in cortical cell culture is calcium dependentNeuroscience Letters, 1985
- Determination of monoamines in brain nuclei by high performance liquid chromatography with electrochemical detection : Young vs. middle aged ratsLife Sciences, 1984
- Substitution of extracellular sodium ions blocks the voltage-dependent decrease of input conductance evoked by L-aspartateCanadian Journal of Physiology and Pharmacology, 1984
- Topographic changes in high-affinity glutamate uptake in the cat red nucleus, substantia nigra, thalamus, and caudate nucleus after lesions of sensorimotor cortical areasExperimental Neurology, 1983
- Aspartate and glutamate induced reductions in extracellular free calcium and sodium concentration in area CA1 of ‘in vitro’ hippocampal slices of ratsNeuroscience Letters, 1983
- A quantitative electron microscopic study on synapse formation in dissociated fetal rat cerebral cortex in vitroDevelopmental Brain Research, 1981
- Turning behaviour and catalepsy after injection of excitatory amino acids into rat substantia nigraNeuroscience Letters, 1981
- Hyperactivity following injection of a glutamate agonist and 6, 7-ADTN into rat nucleus accumbens and its inhibition by THIPLife Sciences, 1981
- Ionic mechanisms underlying the depolarization of L-glutamate on rat and human spinal neurones in tissue cultureCellular and Molecular Life Sciences, 1973