Nucleotide sequences of the 5S ribosomal RNA genes and their adjacent regions in Schizosaccharomyces pombe
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (23), 6429-6437
- https://doi.org/10.1093/nar/9.23.6429
Abstract
The organization of 5S ribosomal RNA (rRNA) genes in the genome of Schizosaccharomyces pombe has been investigated by restriction and hybridization analyses. The 5S rRNA genes were not linked to the other three species of rRNA genes which formed a repeating unit of 6.9 megadaltons, but located in other regions surrounded by heterogeneous sequences. The 5S rRNA gene organization in S. pombe is therefore different from those in other yeasts; Saccharomyces cerevisiae and Torulopsis utilis. Four restriction segments of different sizes each containing a single 5S rRNA gene were cloned on a bacterial plasmid, and the sequences in and around the RNA coding regions were determined. In the RNA coding regions, the sequences in four clones were identical with an exception that one residue has been substituted in one clone. In the flanking regions, the sequences were extremely rich in the AT-content and highly heterogeneous. The sequences were also markedly different from those in the corresponding regions of the other two yeasts. THe presence of T-clusters in the regions immediately after the RNA coding sequences was only notable homology among the four clones and the other two yeasts.Keywords
This publication has 16 references indexed in Scilit:
- Nucleotide sequence of the kanamycin resistance transposon Tn903Journal of Molecular Biology, 1981
- [9] Specific labeling of 3′ termini of RNA with T4 RNA ligaseMethods in Enzymology, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- On spacersCell, 1979
- Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.Journal of Biological Chemistry, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences, 1975
- Studies on the Nucleotide Sequence of Pseudouridine-containing 5 S RNA from Saccharomyces cerevisiaeThe Journal of Biochemistry, 1974
- Nucleotide sequence of 5 S RNA from Torulopsis utilisFEBS Letters, 1974