Leucine-58 in the putative 5th helical region of human interleukin (IL)-6 is important for activation of the IL-6 signal transducer, gp130
- 7 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 369 (2-3), 187-191
- https://doi.org/10.1016/0014-5793(95)00741-q
Abstract
A model of the tertiary structure of human IL-6, derived from the crystal-structure of granulocyte-colony stimulating factor, reveals a 5th helical region in the loop between the first and second α-helix. To investigate the importance of this region for biological activity of IL-6, residues Glu-52, Ser-53, Ser-54, Lys-55, Glu-56, Leu-58 and Glu-60 were individually replaced by alanine. IL-6·Leu-58Ala displayed a 5-fold reduced biological activity on the IL-6-responsive human cell lines XG-1 and A375. This reduction in bioactivity was shown to be due to a decreased capacity of the mutant protein to trigger IL-6 receptor-α-chain-dependent binding to the IL-6 signal transducer, gp130Keywords
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