Characterization of Two Cytosolic Diacylglycerol Kinase Forms
- 1 April 1993
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 60 (4), 1212-1219
- https://doi.org/10.1111/j.1471-4159.1993.tb03279.x
Abstract
Two forms of rat brain cytosolic diacylglycerol kinase (EC2.7.1.107) were separated by heparin-agarose column chromatography. These forms, designated DGK-I and DGK-II, were not interconvertible as determined by rechromatography. DGK-I and DGK-II had respective molecular masses of 88 and 180 kDa, as measured by Sepharose 6B chromatography. Both forms preferred diacylglycerol over monoacylglycerol and were insensitive to R59022. DGK-II, but not DGK-I, was activated by an activator substance prepared from chicken egg yolk. DGK-II was activated by a rat brain cytosolic activator and was exclusively sensitive to 5'-AMP-mediated inactivation. Further studies revealed that these two forms had the following distinct characteristics: (a) substrate specificity, (b) inhibition by heparin, (c) sensitivity to lysine-containing polyamino acids, and (d) responses to different phospholipids. In general, DGK-II was more responsive to various inhibitors and activators, making it a prime candidate for a regulatable enzyme.Keywords
This publication has 25 references indexed in Scilit:
- Cytosolic rat brain synapsin I is a diacylglycerol kinase.Proceedings of the National Academy of Sciences, 1991
- Sustained increase in 1,2-diacylglycerol precedes DNA synthesis in epidermal-growth-factor-stimulated fibroblasts. Evidence for stimulated phosphatidylcholine hydrolysisBiochemical Journal, 1990
- Diacylglycerol kinase: a key modulator of signal transduction?Trends in Biochemical Sciences, 1990
- Diacylglycerol-induced translocation of diacylglycerol kinase: use of affinity-purified enzyme in a reconstitution system.Proceedings of the National Academy of Sciences, 1986
- Phosphorylation of pig brain diacylglycerol kinase by endogenous protein kinaseFEBS Letters, 1986
- Properties of diacylglycerol kinase purified from bovine brainLipids, 1986
- Utilization of diacylglycerol in phospholipid bilayers by pig brain diacylglycerol kinase and Rhizopus arrhizus lipase.Journal of Biological Chemistry, 1984
- Enzymes of Glycerolipid Synthesis in EukaryotesAnnual Review of Biochemistry, 1980
- Properties of Microsomal and Soluble Diacylglycerol Kinase in Rat LiverEuropean Journal of Biochemistry, 1978
- The selective loss of lysophospholipids in some commonly used lipid-extraction proceduresAnalytical Biochemistry, 1974