Separation of Replicative Intermediate from Single-stranded Ribonucleic Acid by Sedimentation at Low Ionic Strength
- 1 April 1969
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 3 (4), 445-447
- https://doi.org/10.1128/jvi.3.4.445-447.1969
Abstract
By sedimentation in 2.5 × 10−4m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease.Keywords
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