Preparation of Desoxyribonucleosides from Thymonucleic Acid.
- 1 January 1950
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 4 (7), 1047-1053
- https://doi.org/10.3891/acta.chem.scand.04-1047
Abstract
The crude desoxyribosides were prepared by enzymatic hydrolysis of thymonucleic acid (II) and were purified by elution from a cation exchanger (Dowex 50) (III), first using 01 [image] NH4 acetate buffer of pH 3.9 followed by 5% NH4OH. The nucleosides were further purified by crystallization. Before crystallization of desoxycytidine, it was rerun on III with 0.5 [image] HC1 as eluting agent. When only small quantities of II are available, a chromatogram on starch developed with butanol-water could be used after running on III instead of the crystallization step.This publication has 5 references indexed in Scilit:
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