An enrichment method for heme-less mutants of Saccharomyces cerevisiae based on photodynamic propeties of Zn-protoporphyrin
- 1 July 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 178 (3), 713-716
- https://doi.org/10.1007/bf00337883
Abstract
A new and widely applicable technique has been elaborated for enrichment of mutants of Saccharomyces cerevisiae deficient in porphyrin and heme synthesis. The method is based on selective photooxidative killing of the wild-type cells, sensitized by Zn-protoporphyrin synthesized and accumulated in wild-type cells under defined conditions. Using a single cycle of selection, heme-deficient mutants have been obtained with a frequency of 2–2.3%.Keywords
This publication has 17 references indexed in Scilit:
- On the mode of cytotoxic action of photoactivated porphyrinsBiochemical Pharmacology, 1979
- Etude de l'inhibition du système respiratoire des levures par la Zn-protoporphyrine en fonction de la photolyse de cette substanceBiochimie, 1977
- Changes in the activities of the protoheme-synthesizing system during the growth of yeast under different conditionsArchives of Biochemistry and Biophysics, 1977
- Hemoproteid formation in yeastMolecular Genetics and Genomics, 1974
- Porphyrin mutants of Saccharomyces cerevisiae: Correlated lesions in sterol and fatty acid biosynthesisBiochemical and Biophysical Research Communications, 1974
- Isolation of pleiotropic yeast mutants requiring ergosterol for growthBiochemical and Biophysical Research Communications, 1973
- Effet de l'accumulation de Zn-protoporphyrine par la cellule de levure sur la synthese et le fonctionnement de son systeme respiratoireBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1971
- Étude de l'activité ferrochélatase des mitochondries de levureBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- Lethal effect of visible light on a mutant strain of haploid yeast. II. Absorption and action spectraArchives of Biochemistry and Biophysics, 1957
- Lethal effect of visible light on a mutant strain of haploid yeast. I. General dependenciesArchives of Biochemistry and Biophysics, 1957