Newborn screening by DNA analysis of dried blood spots
- 1 May 1989
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 82 (2), 134-136
- https://doi.org/10.1007/bf00284045
Abstract
Amplification of DNA recovered from a dried blood spot was used to genotype individuals with sickle cell disease, sickle cell carriers, and controls. A single 200-μl blood spot applied to a filter paper provides sufficient material for more than 20 genetic analyses. In addition, the stability of the DNA is such that adequate material for amplification can be isolated from dried blood spots up to a year following collection. The DNA analysis methods described in this study could be applied to large-scale screening of newborns for genetic disorders.This publication has 14 references indexed in Scilit:
- Mitochondrial DNA sequences from a 7000-year old brainNucleic Acids Research, 1988
- Short but faithful pieces of ancient DNANature, 1988
- Diagnosis of Sickle Cell Anemia and β-Thalassemia with Enzymatically Amplified DNA and Nonradioactive Allele-Specific Oligonucleotide ProbesNew England Journal of Medicine, 1988
- Polymerase chain reaction.1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Detection of sickle cell anaemia and thalassaemiasNature, 1987
- DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screeningHuman Genetics, 1987
- Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic SequencesScience, 1986
- Analysis of Restriction Fragment Length Polymorphisms in Deoxyribonucleic Acid (DNA) Recovered from Dried BloodstainsJournal of Forensic Sciences, 1986
- Genetic ScreeningPublished by Springer Nature ,1973