Excision and Recombination of Adenovirus DNA Fragments in Escherichia coli
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 208-210
- https://doi.org/10.1126/science.322283
Abstract
A hybrid between bacteriophage .lambda. genome and the adenovirus type 2(Ad2) EcoRI-B DNA fragment was constructed in vitro. During growth in E. coli, recombination occurs within multiple adenovirus DNA fragments of the .lambda. vector. This recombination can be intra- or intermolecular. The rapidity of the conversion toward a population in which monomers are predominant suggests that intramolecular recombination is favored. The bacterial recombination system apparently works normally for DNA from a eukaryotic virus.This publication has 5 references indexed in Scilit:
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- Mapping of Adenovirus 2 RNA Sequences in Lytically Infected Cells and Transformed Cell LinesPublished by Cold Spring Harbor Laboratory ,1974