Rapid separation of mouse T and B lymphocytes using wheat germ agglutinin
- 1 April 1979
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 99 (1), 95-99
- https://doi.org/10.1002/jcp.1040990111
Abstract
A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 μg/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from monodisperse T cells by gravity sedimentation and subsequently dissociated into single cells by treatment with N-acetylglucosamine (NAG). Immunocytochemical analyses and mitogenic assays indicate approximately 10-15% cross contamination of the resultant B and T cell fractions. The separation procedure is not only convenient and rapid but also allows the simultaneous recovery of viable T and B cells from the same spleen preparation.Keywords
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