Structural Study of the Carbohydrate Moiety of Bovine Pancreatic Ribonuclease B1
- 1 July 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 88 (1), 51-58
- https://doi.org/10.1093/oxfordjournals.jbchem.a132966
Abstract
The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-β-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatography, and their structures were studied by sequential exoglycosidase digestion, methylation study, periodate oxidation and acetolysis. The results show that bovine pancreatic ribonuclease B has one of the following sugar chains as asparagine-linked carbohydrate.This publication has 15 references indexed in Scilit:
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