Two inhibitors of neutrophil chemotaxis are produced by hyperimmunoglobulin E recurrent infection syndrome mononuclear cells exposed to heat-killed staphylococci

Abstract

Mononuclear cells from normal volunteers and from patients with the hyper-IgE recurrent infection syndrome (HIE) were cultured for 18 h with and without opsonized, heat-killed Staphylococcus aureus (OS). The supernatants from normal mononuclear cell cultures without OS revealed no inhibitory activity for neutrophil chemotaxis; those from HIE patients revealed the 61,000 dalton factor. When normal cells were cultured with OS, they produced a proteinaceous, 56.degree. C-stable, 30,000-45,000 dalton factor which preferentially inhibited neutrophil vs. monocyte chemotaxis. When HIE cells were exposed to OS, they produced the same 30,000-45,000 dalton factor as normal cells and the 1,000-fold dilutions of supernatants from cultures of normal mononuclear cells with OS revealed mean production of 7.8 .+-. 5.4% inhibition of chemotaxis; assay of 1000-fold dilutions of supernatants from cultures of HIE mononuclear cells (spontaneously producing the 61,000 dalton factor) with OS revealed a 26.6 .+-. 3.6% inhibition (P < 0.02). Thus, in short-term culture, normal and HIE mononuclear cells produce an inhibitor of neutrophil chemotaxis when exposed to particulate heat-killed staphylococci; HIE cells produce qualitatively and quantiatively more inhibitory activity.