Direct solid-phase 125I radioimmunoassay of serum cortisol.
- 1 September 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (9), 1595-1598
- https://doi.org/10.1093/clinchem/24.9.1595
Abstract
We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.This publication has 4 references indexed in Scilit:
- New, advantageous approach to the direct radioimmunoassay of cortisol.Clinical Chemistry, 1977
- An 125I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured.Clinical Chemistry, 1976
- Immobilized enzyme-based flowing-stream analyzer for measurement of penicillin in fermentation brothsAnalytical Chemistry, 1976
- Direct radioimmunoassay of plasma cortisolClinica Chimica Acta; International Journal of Clinical Chemistry, 1976