Contractile basis of ameboid movement. VII. Aequorin luminescence during ameboid movement, endocytosis, and capping.
Open Access
- 1 August 1980
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 86 (2), 599-607
- https://doi.org/10.1083/jcb.86.2.599
Abstract
Aequorin luminescence has been utilized to determine the spatial and temporal fluctuations of the free calcium ion concentration [Ca++] in Chaos carolinensis during ameboid movement, pinocytosis, and capping. The [Ca++] increases above approximately 10(-7) M during normal ameboid movement. Three types of luminescent signals are detected in cells: continuous luminescence, spontaneous pulses, and stimulated pulses. Continuous luminescence is localized in the tails of actively motile cells, and spontaneous pulses occur primarily over the anterior regions of cells. We are sometimes able to correlate the spontaneous pulses with extending pseudopods, whereas stimulated pulses are induced by mechanical damage, electrical stimulation, concanavalin A-induced capping, and pinocytosis. The localization of both distinct actin structures and sites where [Ca++] increases suggests cellular sites of contractile activity. The independent evidence from localizing actin structures and the distribution of [Ca++] can also be viewed in relation to the solation-contraction coupling hypothesis defined in vitro.This publication has 54 references indexed in Scilit:
- Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.The Journal of cell biology, 1980
- Relations between ameboid movement and membrane-controlled electrical currents.The Journal of general physiology, 1977
- The contractile basis of amoeboid movement *1IV. The viscoelasticity and contractility of amoeba cytoplasm in vivoExperimental Cell Research, 1977
- Control of Contractility in Spirostomum by Dissociated Calcium IonsThe Journal of general physiology, 1970
- Plasma Membrane: Substructural Changes Correlated with Electrical Resistance and PinocytosisScience, 1967
- Some Ionic and Bioelectric Properties of the Ameba Chaos chaos The Journal of general physiology, 1965
- A STUDY OF PHAGOCYTOSIS IN THE AMEBA CHAOS CHAOS The Journal of cell biology, 1965
- A study on electrophysiological properties of carnivorous amoebaeJournal of Cellular and Comparative Physiology, 1964
- Pseudopod initiation and membrane depolarisation in Amoeba proteusExperimental Cell Research, 1962
- MICRURGICAL STUDIES IN CELL PHYSIOLOGYThe Journal of general physiology, 1928