Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli
- 1 March 1983
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 39 (3), 1167-1174
- https://doi.org/10.1128/iai.39.3.1167-1174.1983
Abstract
A gene encoding a heat-stable enterotoxin (ST) from an Escherichia coli strain isolated from a human with diarrhea was cloned and characterized by nucleotide sequence analysis. The gene was found to be partially homologous to a previously characterized ST gene from an E. coli strain of bovine origin. Hybridization studies showed that most ST-producing strains of E. coli isolated from humans with diarrhea possess genes highly homologous to either the ST gene from the bovine strain or the ST gene characterized in the present study.This publication has 27 references indexed in Scilit:
- Identification of Enterotoxigenic Escherichia coli by Colony Hybridization Using Three Enterotoxin Gene ProbesThe Journal of Infectious Diseases, 1982
- A Study of the Dissemination of Tn1681: A Bacterial Transposon Encoding a Heat-stable Toxin among Enterotoxigenic Escherichia coli IsolatesCold Spring Harbor Symposia on Quantitative Biology, 1981
- Detection of Enterotoxigenic Escherichia coli by DNA Colony HybridizationThe Journal of Infectious Diseases, 1980
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in Enzymology, 1980
- [46] Recovery of DNA from gelsMethods in Enzymology, 1980
- The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1Nature, 1979
- [16] Plasmids of Escherichia coli as cloning vectorsMethods in Enzymology, 1979
- Control of Transcription TerminationAnnual Review of Biochemistry, 1978
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Test for Escherichia coli Enterotoxin Using Infant Mice: Application in a Study of Diarrhea in Children in HonoluluThe Journal of Infectious Diseases, 1972