Mastoparan inhibits phosphoinositide hydrolysis via pertussis toxin‐intensive G‐protein in human astrocytoma cells
- 15 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (1), 91-94
- https://doi.org/10.1016/0014-5793(90)80074-s
Abstract
Mastoparan inhibited [3H]inositol phosphate accumulation induced by carbachol as well as cyclic AMP accumulation induced by isoproterenol in 1321N1 human astrocytoma cells. Mastoparan inhibited GTPγS-induced, but not Ca2+-induced, [3H]inositol phosphate accumulation in membrane preparations with an IC 50 of approximately 10 μM. The inhibitory effect of mastoparan on carbachol-induced [3pH]inositol phosphate accumulation was resistant to pertussis toxin (IAP) treatment in intact cells. These results suggest that mastoparan inhibits phospholipase C in human astrocytoma cells via a GTP binding protein, which is not a substrate for IAP.Keywords
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