II. Mitt.: Zur Immunmorphologie der „Plaque-bildenden“ Milchflecken im Mäuseomentum

Abstract

Mice were immunized by intraperitoneal injection of sheep erythrocytes. The morphology of the cells which consecutively accumulate in non-vascularized milky spots has been studied on stained omental spreads. Macrophages, lymphocytes, large pyroninophilic cells, plasma cells, mast cells and eosinophilic granulocytes were found to be present in varying numbers depending on the stage of the immune process. Mast cells and eosinophilic granulocytes appeared in appreciable numbers not before several weeks after immunization. Milky spots - caused by intraperitoneal stimulation with two morphologically different types of erythrocytes (pigeon-sheep; camel-sheep) were shown to produce antibodies against both cell types. This was demonstrated with a modification of the Jerne - plaque-technique. By contrast, single cells, isolated from doubly-producing milky spots released antibody directed against either sheep or pigeon resp. camel erythrocytes. Thus far no doubly producing single cells have been found.