A New Colorimetric Reagent for Micro Determination of Ammonia.

Abstract

Ammonia is separated by micro-diffusion (Conway) and oxidized by hypobromite, the excess of the latter being measured colorimetrically by its power to decolorize pheno-safranin. The range is .05 to .5 micromoles ammonia. Application to blood and tissues is described. For non-protein nitrogen, Koch-McMeekin digestion is followed by colorimetric measurement without microdiffusion.